PMID- 19542345
OWN - NLM
STAT- MEDLINE
DA  - 20090727 
DCOM- 20090921
IS  - 1098-5336 (Electronic)
VI  - 75
IP  - 15
DP  - 2009 Aug
TI  - Comparative genome-wide transcriptional profiling of Azorhizobium caulinodans
      ORS571 grown under free-living and symbiotic conditions.
PG  - 5037-46
AB  - The whole-genome sequence of the endosymbiotic bacterium Azorhizobium
      caulinodans ORS571, which forms nitrogen-fixing nodules on the stems and roots
      of Sesbania rostrata, was recently determined. The sizes of the genome and
      symbiosis island are 5.4 Mb and 86.7 kb, respectively, and these sizes are the
      smallest among the sequenced rhizobia. In the present study, a whole-genome
      microarray of A. caulinodans was constructed, and transcriptomic analyses were
      performed on free-living cells grown in rich and minimal media and in bacteroids
      isolated from stem nodules. Transcriptional profiling showed that the genes
      involved in sulfur uptake and metabolism, acetone metabolism, and the
      biosynthesis of exopolysaccharide were highly expressed in bacteroids compared
      to the expression levels in free-living cells. Some mutants having Tn5
      transposons within these genes with increased expression were obtained as
      nodule-deficient mutants in our previous study. A transcriptomic analysis was
      also performed on free-living cells grown in minimal medium supplemented with a
      flavonoid, naringenin, which is one of the most efficient inducers of A.
      caulinodans nod genes. Only 18 genes exhibited increased expression by the
      addition of naringenin, suggesting that the regulatory mechanism responding to
      the flavonoid could be simple in A. caulinodans. The combination of our
      genome-wide transcriptional profiling and our previous genome-wide mutagenesis
      study has revealed new aspects of nodule formation and maintenance.
AD  - Biotechnology Research Center, University of Tokyo, Yayoi, Bunkyo-ku, Japan.
FAU - Tsukada Shuhei 
AU  - Tsukada S 
FAU - Aono Toshihiro 
AU  - Aono T 
FAU - Akiba Noriko 
AU  - Akiba N 
FAU - Lee Kyung-Bum 
AU  - Lee KB 
FAU - Liu Chi-Te 
AU  - Liu CT 
FAU - Toyazaki Hiroki 
AU  - Toyazaki H 
FAU - Oyaizu Hiroshi
AU  - Oyaizu H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090619
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0(Flavanones)
RN  - 480-41-1(naringenin)
SB  - IM
MH  - Azorhizobium caulinodans/growth & development/*physiology
MH  - Flavanones/metabolism
MH  - *Gene Expression Profiling
MH  - *Gene Expression Regulation, Bacterial
MH  - Metabolic Networks and Pathways/genetics
MH  - Oligonucleotide Array Sequence Analysis
MH  - Sesbania/microbiology
MH  - *Symbiosis
MH  - Transcriptional Activation
PMC - PMC2725507 [Available on 02/01/10]
OID - NLM:PMC2725507 [Available on 02/01/10]
EDAT- 2009/06/23 09:00
MHDA- 2009/09/22 06:00
CRDT- 2009/06/23 09:00
PHST- 2009/06/19 00:00[aheadofprint]
PHST- 2010/02/01 00:00[pmc-release]
AID - AEM.00398-09[pii]
AID - 10.1128/AEM.00398-09[doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2009 Aug ; 75(15):5037-46 Epub 2009 06 19.