PMID- 19566440
OWN - NLM
STAT- MEDLINE
DA  - 20091209 
DCOM- 20100203
IS  - 1937-335X (Electronic)
VI  - 15
IP  - 12
DP  - 2009 Dec
TI  - In vitro-cultured human islet cell monolayers: stemness markers and insulin
      recovery upon streptozotocin exposure.
PG  - 3931-42
AB  - We have investigated long-term in vitro-cultured human islet-derived cell
      monolayers (CM) to determine their suitability as source of newly generated
      beta-cells. Initial loss of the islet three-dimensional architecture resulted in
      rearrangement of pancreatic hormone and key transcriptional factor expression,
      or decreased insulin secretion, or declined glucose-stimulated insulin release,
      reflecting reversal to an immature precursor stage. However, upon exposure to
      several streptozotocin (STZ) concentrations, CM showed glucose-stimulated
      insulin release recovery and an increased synthesis of insulin mRNA. Possibly,
      this outcome could derive from eventual "stemness" properties of such cell
      populations, unfolded by subtoxic STZ-induced CM damage. To test this
      hypothesis, we have examined messenger and protein expression of embryonic stem
      cell markers (Nanog, Sox-2, Oct-4A), which varied in CM versus control whole
      human islets. Moreover, different STZ concentrations were shown to modulate
      marker expression in CM. All together our data preliminarily seem to indicate
      that CM may generate reconstituted insulin-producing cells likely due to their
      intrinsic, preexisting stemness properties.
AD  - Section of Internal Medicine and Endocrine and Metabolic Sciences (Di.M.I.), Department of Internal Medicine, University of Perugia, Perugia, Italy.
FAU - Montanucci Pia 
AU  - Montanucci P 
FAU - Basta Giuseppe 
AU  - Basta G 
FAU - Calafiore Riccardo
AU  - Calafiore R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Tissue Eng Part A
JT  - Tissue engineering. Part A
JID - 101466659
RN  - 0(Biological Markers)
RN  - 0(RNA, Messenger)
RN  - 11061-68-0(Insulin)
RN  - 18883-66-4(Streptozocin)
RN  - 50-99-7
SB  - IM
MH  - Adult
MH  - Apoptosis/drug effects
MH  - Biological Markers/metabolism
MH  - Cell Proliferation/drug effects
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Gene Expression Regulation/drug effects
MH  - Glucose/pharmacology
MH  - Humans
MH  - Insulin/*metabolism/secretion
MH  - Islets of Langerhans/*cytology/drug effects/*metabolism/secretion
MH  - Phenotype
MH  - RNA, Messenger/genetics/metabolism
MH  - Stem Cells/cytology/drug effects/*metabolism
MH  - Streptozocin/*pharmacology
EDAT- 2009/07/02 09:00
MHDA- 2010/02/04 06:00
CRDT- 2009/07/02 09:00
AID - 10.1089/ten.TEA.2009.0124[doi]
PST - ppublish
SO  - Tissue Eng Part A. 2009 Dec ; 15(12):3931-42.