PMID- 19568771
OWN - NLM
STAT- MEDLINE
DA  - 20090821 
DCOM- 20090827
IS  - 1617-4623 (Electronic)
VI  - 282
IP  - 3
DP  - 2009 Sep
TI  - Transgene constructs lacking transcription termination signal induce efficient
      silencing of endogenous targets in Arabidopsis.
PG  - 319-28
AB  - Efficient gene silencing tools are extremely important for genomics and
      biotechnology applications. A number of aberrant RNA species induce silencing of
      homologous genes; however, only the inverted repeat RNA is widely utilized for
      targeted gene silencing in plants. Development of additional silencing
      constructs would not only provide an alternative technology for single targets,
      it may also serve as a simpler platform for multi-target silencing. The present
      study found that aberrantly terminated sense transcripts serve as efficient
      inducers of gene silencing. A construct initially designed to carry out
      homologous recombination into the Phytochrome A locus of Arabidopsis induced
      instead a high rate of Phytochrome A silencing, resulting in a strong mutant
      phenotype. Attempts to decipher the silencing determinant of the construct
      revealed that absence of a proper transcription termination signal contributed
      to the silencing efficiency of the construct. Furthermore, silencing of an
      additional gene, Phytochrome B, was conferred by a separate construct of a
      similar design. Proper placement of a transcription termination signal in the
      silencing construct abolished its silencing ability. Therefore, transcription of
      aberrantly terminated transcripts was deduced to be the cause of the elevated
      rate of silencing observed in transgenic lines. These transcripts could be
      produced either by direct transcription or read-through transcription. Since
      silencing resulted in strong mutant phenotypes in the majority of transgenic
      lines, terminator-less constructs are proposed to serve as efficient tools for
      knocking out endogenous targets.
AD  - Department of Crop, Soil and Environmental Sciences, University of Arkansas, Fayetteville, USA.
FAU - Nicholson Scott J 
AU  - Nicholson SJ 
FAU - Srivastava Vibha
AU  - Srivastava V
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090701
PL  - Germany
TA  - Mol Genet Genomics
JT  - Molecular genetics and genomics : MGG
JID - 101093320
RN  - 0(Phytochrome A)
RN  - 136250-22-1(Phytochrome B)
SB  - IM
MH  - Arabidopsis/*genetics
MH  - Base Sequence
MH  - *Gene Silencing
MH  - Phytochrome A/*genetics
MH  - Phytochrome B/*genetics
MH  - Plants, Genetically Modified
MH  - Promoter Regions, Genetic
MH  - *Terminator Regions, Genetic
MH  - Transcription, Genetic
MH  - Transgenes
EDAT- 2009/07/02 09:00
MHDA- 2009/08/28 09:00
CRDT- 2009/07/02 09:00
PHST- 2009/01/29 00:00[received]
PHST- 2009/06/10 00:00[accepted]
PHST- 2009/07/01 00:00[aheadofprint]
AID - 10.1007/s00438-009-0467-1[doi]
PST - ppublish
SO  - Mol Genet Genomics. 2009 Sep ; 282(3):319-28 Epub 2009 07 01.