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次世代暗号技術「量子暗号通信」大規模開発プロジェクト始動へ (NHK)

「量子暗号通信」と呼ばれる次世代の暗号技術の世界展開を目指して、大手企業や大学などが参加する大がかりな開発プロジェクトが、今月始まることが分かりました。 インタ...

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Constructing Physical and Genomic Maps for Puccinia striiformis f. sp. tritici, the Wheat Stripe Rust Pathogen, by Comparing Its EST Sequences to the Genomic Sequence of P. graminis f. sp. tritici, the Wheat Stem Rust Pathogen.

著者 Ma J , Chen X , Wang M , Kang Z
Comp Funct Genomics.2009 ; ():302620.
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College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China.

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The wheat stripe rust fungus, Puccinia striiformis f. sp. tritici (Pst), does not have a known alternate host for sexual reproduction, which makes it impossible to study gene linkages through classic genetic and molecular mapping approaches. In this study, we compared 4,219 Pst expression sequence tags (ESTs) to the genomic sequence of P. graminis f. sp. tritici (Pgt), the wheat stem rust fungus, using BLAST searches. The percentages of homologous genes varied greatly among different Pst libraries with 54.51%, 51.21%, and 13.61% for the urediniospore, germinated urediniospore, and haustorial libraries, respectively, with an average of 33.92%. The 1,432 Pst genes with significant homology with Pgt sequences were grouped into physical groups corresponding to 237 Pgt supercontigs. The physical relationship was demonstrated by 12 pairs (57%), out of 21 selected Pst gene pairs, through PCR screening of a Pst BAC library. The results indicate that the Pgt genome sequence is useful in constructing Pst physical maps.
PMID: 20169145 [PubMed]
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