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腸内ウイルス由来の酵素で原因菌を破壊 大阪市大と東大の研究チーム (毎日新聞)

[PR] 腸内細菌のウイルス感染状況を網羅的に調べることで、偽膜性腸炎の原因菌を破壊する抗菌物質を見つけ出す方法を開発したと、大阪市大と東京大の研究チームが発表...

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A modified protocol to prepare seed-free starting solutions of amyloid-β (Aβ)₁₋₄₀ and Aβ₁₋₄₂ from the corresponding depsipeptides.

著者 Beeg M , Stravalaci M , Bastone A , Salmona M , Gobbi M
Anal Biochem.2011 Apr 15 ; 411(2):297-9.
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Department of Biochemistry and Molecular Pharmacology, Instituto di Ricerche Farmacologiche "Mario Negri", 20156 Milano, Italy. marten.beeg@chem.ethz.ch

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Preparing reliable, seed-free stock solutions of the highly amyloidogenic peptides amyloid-β (Aβ) is difficult. Besides the formation of aggregates during synthesis and storage, dissolution of the peptide is a critical step because vortexing can induce aggregation. To overcome this, synthesis of the more water-soluble depsi-Aβ(1-42) peptide, from which the native sequence is easily obtained, has been suggested. We further refined this technique, including a cutoff filtration step and switching the depsipeptide in basic conditions, to stabilize the formed native peptide. The obtained solutions of native Aβ(1-40) and Aβ(1-42) peptides were homogeneous and aggregate free, as indicated by thioflavin T and circular dichroism analysis.
PMID: 21185802 [PubMed - indexed for MEDLINE]
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