絞り込み

16514

広告

“賛否両論の登山家”栗城史多さんとは何者だったのか | 文春オンライン

登山家の栗城史多さんがエベレストで亡くなった。これを書いている5月22日現在では、死因などについての詳しい情報が入ってきていないので、事故についてはなにもわから...

  1. [医学] LRRK2キナーゼの結核菌助長...
  2. 東急不動産が再エネ事業に本腰、全国で40...
  3. 高い場所にいる人ほど、リスクが高い決断を...
  4. 6月22日にプライベートセミナー開催 ビ...

ニュース一覧

In vitro reconstitution of SV40 particles that are composed of VP1/2/3 capsid proteins and nucleosomal DNA and direct efficient gene transfer.

著者 Enomoto T , Kukimoto I , Kawano MA , Yamaguchi Y , Berk AJ , Handa H
Virology.2011 Sep 12 ; ():.
この記事をPubMed上で見るPubMedで表示
この記事をGoogle翻訳上で見る Google翻訳で開く

Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa 226-8501, Japan.

スターを付ける スターを付ける     (323view , 0users)

Full Text Sources

Other Literature Sources

SV40 is comprised of the viral minichromosome and the capsid proteins VP1, VP2, and VP3. Complete reconstitution of SV40 virions in vitro remains a challenge. Here we describe in vitro reconstitution of SV40 particles that contain ~5-kb circular nucleosomal DNA with hyperacetylated histones and are encapsidated in a coat composed of VP1, VP2, and VP3, closely mimicking the characteristics of authentic SV40 virions. When inoculated into mammalian cells, VP1/2/3 particles containing nucleosomal DNA carrying a reporter gene yielded a significantly higher level of gene expression than VP1-only particles containing the corresponding naked DNA. The elevated gene expression resulted mainly from enhanced association of the particles with the cell surface and from facilitation of subsequent uptake into cells. Thus, the in vitro reconstitution system reported here should be useful for the elucidation of Polyomaviridae assembly mechanisms and for the development of novel carriers for gene delivery.
PMID: 21917285 [PubMed - as supplied by publisher]
印刷用ページを開く Endnote用テキストダウンロード