絞り込み

16391

広告

Antisense morpholino targeting just upstream from a poly(A) tail junction of maternal mRNA removes the tail and inhibits translation.

著者 Wada T , Hara M , Taneda T , Qingfu C , Takata R , Moro K , Takeda K , Kishimoto T , Handa H
Nucleic Acids Res.2012 Aug 16 ; ():.
この記事をPubMed上で見るPubMedで表示
この記事をGoogle翻訳上で見る Google翻訳で開く

Division of Molecular and Cellular Biology, Department of Supramolecular Biology, Graduate School of Nanobioscience, Yokohama City University, Tsurumi-ku, Yokohama, Kanagawa, 230-0045, Graduate School of Bioscience and Biotechnology, Yoshindo Inc. Fuchu-m

スターを付ける スターを付ける     (308view , 0users)
  • このエントリーをはてなブックマークに追加

Full Text Sources

Miscellaneous

Molecular Biology Databases

Other Literature Sources

Gene downregulation by antisense morpholino oligonucleotides (MOs) is achieved by either hybridization around the translation initiation codon or by targeting the splice donor site. In the present study, an antisense MO method is introduced that uses a 25-mer MO against a region at least 40-nt upstream from a poly(A) tail junction in the 3'-untranslated region (UTR) of maternal mRNA. The MO removed the poly(A) tail and blocked zebrafish cdk9 (zcdk9) mRNA translation, showing functional mimicry between miRNA and MO. A PCR-based assay revealed MO-mediated specific poly(A) tail removal of zebrafish mRNAs, including those for cyclin B1, cyclin B2 and tbp. The MO activity targeting cyclins A and B mRNAs was validated in unfertilized starfish oocytes and eggs. The MO removed the elongated poly(A) tail from maternal matured mRNA. This antisense method introduces a new application for the targeted downregulation of maternal mRNAs in animal oocytes, eggs and early embryos.
PMID: 22904086 [PubMed - as supplied by publisher]
印刷用ページを開く Endnote用テキストダウンロード