絞り込み

16506

広告

愛知 新たに幼女がはしか感染 沖縄は患者70人に (NHK)

「はしか」の感染が広がる中、愛知県内では新たに1歳の女の子の感染が確認される一方、沖縄県内では24日までに患者の数が70人に上っています。 愛知県内では、今月沖...

  1. [医学] 活動量計の装着と通話で歩行を促...
  2. 米退役軍人長官候補の指名公聴会延期、「指...
  3. [医学] 出産後12週間(妊産婦4期)の...
  4. 「買収合意」報道も乱れ飛ぶ武田薬品のSh...

ニュース一覧

FgIlv5 is required for branched-chain amino acid biosynthesis and full virulence in Fusarium graminearum.

著者 Liu X , Wang J , Xu J , Shi J
Microbiology.2014 Feb 3 ; ():.
この記事をPubMed上で見るPubMedで表示
この記事をGoogle翻訳上で見る Google翻訳で開く

スターを付ける スターを付ける     (3,812view , 1users)

Full Text Sources

In this study we characterized FgIlv5, a homologue of the Saccharomyces cerevisiae keto-acid reductoisomerase (KARI) from the important wheat head scab fungus Fusarium graminearum. KARI is a key enzyme in the branched-chain amino acids (BCAAs, including leucine, isoleucine and valine) biosynthetic pathway which exists in a variety of organisms from bacteria to fungi and high plants, but not mammals. The FgILV5 deletion mutant ΔFgIlv5-4 failed to grow when the culture medium was nutritionally limited for BCAAs. When grown on PDA plates, ΔFgIlv5-4 exhibited a significant decrease in aerial hyphae formation and red pigmentation. Conidia formation was also blocked in ΔFgIlv5-4. Exogenous addition of 1 mM isoleucine and valine could rescue the defects of mycelial growth and conidial morphogenesis.Cellular stress assays showed that ΔFgIlv5-4 was more sensitive to osmotic and oxidative stresses. In addition, virulence of ΔFgIlv5-4 was dramatically reduced on wheat heads, and a low level of deoxynivalenol (DON) production was detected in ΔFgIlv5-4 in wheat kernels. Taken together, results of this study indicate that FgIlv5 is involved in valine and isoleucine biosynthesis and is required for full virulence in F. graminearum.
PMID: 24493249 [PubMed - as supplied by publisher]
印刷用ページを開く Endnote用テキストダウンロード