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愛知 新たに60代男性1人が新型ウイルス感染確認 (NHK)

愛知県に住む60代の男性が新型コロナウイルスに感染していることが新たに確認されました。男性は、16日感染が確認された患者の知人で、愛知県内で感染が確認されたのは...

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  2. [医学] WNTとNOTCH伝達が頼りの...
  3. 回復患者の血しょう採取 血液製剤で回復傾...
  4. UAE、原発稼働を許可 (デイリースポー...

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EEPD1 Rescues Stressed Replication Forks and Maintains Genome Stability by Promoting End Resection and Homologous Recombination Repair.

著者 Wu Y , Lee SH , Williamson EA , Reinert BL , Cho JH , Xia F , Jaiswal AS , Srinivasan G , Patel B , Brantley A , Zhou D , Shao L , Pathak R , Hauer-Jensen M , Singh S , Kong K , Wu X , Kim HS , Beissbarth T , Gaedcke J , Burma S , Nickoloff JA , Hromas RA
PLoS Genet.2015 Dec ; 11(12):e1005675.
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Replication fork stalling and collapse is a major source of genome instability leading to neoplastic transformation or cell death. Such stressed replication forks can be conservatively repaired and restarted using homologous recombination (HR) or non-conservatively repaired using micro-homology mediated end joining (MMEJ). HR repair of stressed forks is initiated by 5' end resection near the fork junction, which permits 3' single strand invasion of a homologous template for fork restart. This 5' end resection also prevents classical non-homologous end-joining (cNHEJ), a competing pathway for DNA double-strand break (DSB) repair. Unopposed NHEJ can cause genome instability during replication stress by abnormally fusing free double strand ends that occur as unstable replication fork repair intermediates. We show here that the previously uncharacterized Exonuclease/Endonuclease/Phosphatase Domain-1 (EEPD1) protein is required for initiating repair and restart of stalled forks. EEPD1 is recruited to stalled forks, enhances 5' DNA end resection, and promotes restart of stalled forks. Interestingly, EEPD1 directs DSB repair away from cNHEJ, and also away from MMEJ, which requires limited end resection for initiation. EEPD1 is also required for proper ATR and CHK1 phosphorylation, and formation of gamma-H2AX, RAD51 and phospho-RPA32 foci. Consistent with a direct role in stalled replication fork cleavage, EEPD1 is a 5' overhang nuclease in an obligate complex with the end resection nuclease Exo1 and BLM. EEPD1 depletion causes nuclear and cytogenetic defects, which are made worse by replication stress. Depleting 53BP1, which slows cNHEJ, fully rescues the nuclear and cytogenetic abnormalities seen with EEPD1 depletion. These data demonstrate that genome stability during replication stress is maintained by EEPD1, which initiates HR and inhibits cNHEJ and MMEJ.
PMID: 26684013 [PubMed - as supplied by publisher]
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