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Evaluation of strategies for the assembly of diverse bacterial genomes using MinION long-read sequencing.

著者 Goldstein S , Beka L , Graf J , Klassen JL
BMC Genomics.2019 Jan 09 ; 20(1):23.
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Short-read sequencing technologies have made microbial genome sequencing cheap and accessible. However, closing genomes is often costly and assembling short reads from genomes that are repetitive and/or have extreme %GC content remains challenging. Long-read, single-molecule sequencing technologies such as the Oxford Nanopore MinION have the potential to overcome these difficulties, although the best approach for harnessing their potential remains poorly evaluated.
PMID: 30626323 [PubMed - in process]
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