絞り込み

16641

広告

【動画】今日はどうされましたか? (日経BP)

診察室やカンファレンスなどでよく使う医療英語のエッセンスを、今日も楽しく学んでいきましょう。 今日のトレーニングは「最初の3単語」です。診察室でよく使うこのフレ...

  1. 地域包括ケア病棟 (日経BP)
  2. 預かった保険証を紛失! 心当たりをたどる...
  3. 製薬会社からのギフトは処方に影響するか?...
  4. ホルモン受容体陽性進行乳癌へのCDK4/...

ニュース一覧

C-terminal α-synuclein truncations are linked to cysteine cathepsin activity in Parkinson's disease.

著者 McGlinchey RP , Lacy SM , Huffer KE , Tayebi N , Sidransky E , Lee JC
J Biol Chem.2019 May 15 ; ():.
この記事をPubMed上で見るPubMedで表示
この記事をGoogle翻訳上で見る Google翻訳で開く

スターを付ける スターを付ける     (5view , 0users)

Full Text Sources

Miscellaneous

A pathological feature of Parkinson's disease (PD) is Lewy bodies (LBs) composed of α-synuclein (α-syn) amyloid fibrils. α-Syn is 140-amino-acids long protein, but truncated α-syn is enriched in LBs. The proteolytic processes that generate these truncations are not well understood. On the basis of our previous work, we propose that these truncations could originate from lysosomal activity attributable to cysteine cathepsins (Cts). Here, using a transgenic SNCAA53T mouse model, overexpressing the PD-associated α-syn variant A53T, we compared levels of α-syn species in purified brain lysosomes from non-symptomatic mice with those in age-matched symptomatic mice. In the symptomatic mice, antibody epitope mapping revealed enrichment of C-terminal truncations, resulting from CtsB, CtsL, and asparagine endopeptidase. We did not observe changes in individual cathepsin activities, suggesting that the increased levels of C-terminal α-syn truncations are due to the burden of aggregated α-syn. Using LC-MS and purified α-syn, we identified C-terminal truncations corresponding to amino acids 1-122 and 1-90 from the SNCAA53T lysosomes. Feeding rat dopaminergic N27 cells with exogenous α-syn fibrils confirmed that these fragments originate from incomplete fibril degradation in lysosomes. We mimicked these events in situ by asparagine endopeptidase degradation of α-syn fibrils. Importantly, the resulting C-terminally truncated fibrils acted as superior seeds in stimulating α-syn aggregation compared with that of the full length fibrils.  These results unequivocally show that C-terminal α-syn truncations in LBs are linked to Cts activities, promote amyloid formation, and contribute to PD pathogenesis.
PMID: 31092553 [PubMed - as supplied by publisher]
印刷用ページを開く Endnote用テキストダウンロード