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Mutations in the Membrane-Anchored SdhC Subunit Affect Fitness and Sensitivity to Succinate Dehydrogenase Inhibitors in Botrytis cinerea Populations from Multiple Hosts.

著者 Amiri A , Zuniga AI , Peres N
Phytopathology.2019 Sep 10 ; ():.
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Succinate dehydrogenase inhibitors (SDHIs) are key fungicides for managing gray mold, caused by Botrytis cinerea, in numerous crops. Resistance to boscalid, an early generation SDHI, is widespread worldwide and was linked to mutations in the SdhB subunit of the SDH complex. Herein, we report on four simultaneous mutations at codons 85 (G85A), 93 (I93V), 158 (M158V) and 168 (V168I) of the membrane-anchored SdhC subunit of B. cinerea. Isolates without (C-) and with (C+) mutations in the SdhC were found in five surveyed hosts from different U.S. regions. The frequency of C+ genotype was higher, 25-40%, in the tree fruit isolates compared to 12-25% in the small fruit populations. The four SdhC mutations were found in isolates without or with mutations in the SdhB subunit but were more frequent the SdhB-wild type isolates, which suggests a role of SDHIs in selecting for the C- over the C+ genotype. Field C+ isolates exhibited reduced sensitivity to fluopyram and increased sensitivity to boscalid and penthiopyrad in vitro and in vivo. Homology modeling confirmed the positioning of the four mutations in the ubiquinone-binding pocket. Beside the differential sensitivity to the SDHIs, these mutations caused a significant fitness cost in the C+ isolates, i.e. sporulation and increased sensitivity to reactive oxygen species. The presence of Botrytis populations differentially sensitive to the SDHIs suggests increased risks for resistance development but also opens up new perspective for future gray mold management using the SDHI fungicides and others.
PMID: 31502521 [PubMed - as supplied by publisher]
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