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Nanomaterials with intrinsic enzyme-mimicking properties (nanozymes), have been widely considered as artificial enzymes in biomedicine. However, manipulating inorganic nanozyme into multivariant targeted bio-analysis is still challenging because of the insufficient catalytic efficiency and biological blocking effect. Here, we rationally designed a spatially-engineered hollow Janus hybrid nanozyme vector (h-JHNzyme) based on the bifacial modulation of Ag-Au nanocage. The silver face inside the h-JHNzyme serves as an interior gate to promote the enzymatic activity of the Ag-Au nanozyme, while two-dimensional DNAzyme-motif nanobrushes deposited on the exterior surface of the h-JHNzyme endowed the targeting function and tremendously enhanced the peroxidase-mimicking activity. We demonstrated that the spatially-separated modulation of the h-JHNzyme propels it as powerful "all-in-one" enzymatic vector with excellent biocompatibility, specific vectorization, remarkable enzymatic performance and clinical practicability. Further, we programmed it into a stringent catalytic SERS liquid biopsy platform to trace multidimensional tumour-related biomarkers, such as microRNAs and circulating tumour cells, with a limit of detection of fM and single cell level, respectively. The developed enzymatic platform shows great potential to facilitate the reliable quantitative SERS liquid biopsy for on-demand clinical diagnosis.
PMID: 31621282 [PubMed - as supplied by publisher]