絞り込み

16646

広告

手塚治虫さん トキワ荘の天井板に描いた直筆画 豊島区に寄贈 (NHK)

マンガの神様、手塚治虫さんが若き日を過ごしたアパート「トキワ荘」の天井板に描いた貴重な直筆画が東京 豊島区に寄贈されました。来月オープンする「トキワ荘」を復元し...

  1. 女川2号機 審査「合格」…21年度以降の...
  2. 東北電力 女川原発2号機 地元自治体が再...
  3. 無観客ライブを無料配信 会場費などはファ...
  4. 突然の異常行動を伴う頭痛、疑うべき疾患は...

ニュース一覧

Importance of measuring pharmacologically active metabolites of edoxaban: development and validation of an ultra-high-performance liquid chromatography coupled with a tandem mass spectrometry method.

著者 Siriez R , Alpan L , Elasaad K , Devel P , Laloy J , Dogné JM , Douxfils J
J Thromb Thrombolysis.2020 Jan 10 ; ():.
この記事をPubMed上で見るPubMedで表示
この記事をGoogle翻訳上で見る Google翻訳で開く

スターを付ける スターを付ける     (22view , 0users)

Full Text Sources

Although DOACs do not require regular measurements of their blood concentrations, clinical situations may require an assessment of their concentration. Among the factor Xa inhibitors, edoxaban is the only compound for which some metabolites (e.g. edoxaban-M4) are reported to be pharmacologically active. Therefore, their contribution could interfere with assays used for the estimation of edoxaban concentration. In addition, drug interactions may alter the metabolite/parent compound ratio making the sole estimation of edoxaban concentration, a poor assessment of the overall anticoagulation. To develop a validated UHPLC-MS/MS method to quantify simultaneously edoxaban and its more relevant M4-metabolite in human plasma. Electrospray ionization and chromatographic separation were optimized for the simultaneous dosage of edoxaban and edoxaban-M4. The method was validated according to regulatory guidelines for bioanalytical method validation. The total run time was 6 min. The method was validated for calibration curves, precision, accuracy, carry-over, selectivity, matrix effect and short-time stability. This method permits quantification of edoxaban and edoxaban-M4 providing complementary information about the inhibitory effect of this active metabolite in chronometric or chromogenic assays. Although patients treated with edoxaban exhibits usually low concentrations of active metabolites, the measurement of edoxaban-M4 is interesting; especially in case of drug interactions. Indeed, concomitant prescriptions of edoxaban and carbamazepine or rifampicin is frequent and may lead to disturbance of the estimations of edoxaban concentration by chromogenic anti-Xa assays. Therefore, patients are at risk of having inadequate control of anticoagulation supporting the need of measuring the most representative edoxaban metabolite concomitantly to the parent compound.
PMID: 31925664 [PubMed - as supplied by publisher]
印刷用ページを開く Endnote用テキストダウンロード