絞り込み

16610

広告

P(V) Reagents for the Scalable Synthesis of Natural and Modified Nucleoside Triphosphates.

著者 Bala S , Liao JY , Ngor AK , Yik E , Chaput JC
J Am Chem Soc.2019 Jul 12 ; ():.
この記事をPubMed上で見るPubMedで表示
この記事をGoogle翻訳上で見る Google翻訳で開く

スターを付ける スターを付ける     (1view , 0users)

Full Text Sources

Natural and modified nucleoside triphosphates impact nearly every major aspect of healthcare research from DNA sequencing to drug discovery. However, a scalable synthetic route to these molecules has long been hindered by the need for purification by high performance liquid chromatography (HPLC). Here, we describe a fundamental-ly different approach that uses a novel P(V) pyrene pyro-phosphate reagent to generate derivatives that are purified by silica gel chromatography and converted to the desired compounds on scales vastly exceeding those achievable by HPLC. The power of this approach is demonstrated through the synthesis of a broad range of natural and un-natural nucleoside triphosphates (dNTPs and xNTPs) using protocols that are efficient, inexpensive, and operationally straightforward.
PMID: 31298849 [PubMed - as supplied by publisher]
印刷用ページを開く Endnote用テキストダウンロード